Videos and Images
phalloidin stained endothelial cell showing an actin protrusion
phalloidin stained worm showing gut and muscle
An actin reporter expressed in hypodermis of 3 fold embryos shows circumferencial actin bundles
Monolayer of endothelial cells in culture stained for F-actin
Mouse Embryonic Fibroblasts stained for actin (green) and beta-catenin (red) highlighting the cell-cell junctions.
(Image by Megha Vaman Rao)
microtubules in hypodermis of comma stage embryo
integrin beta (pat-3) GFP appearing in dense bodies in muscles of C. elegans
focal adhesions in endothelial cell. A ratiometric image of Zyxin and phosphotyrosine
Hela cells naturally arranged in a circle stained for actin (red) and myosin IIa (green) (image by Megha Rao)
AJM-1::GFP highlights apical junctions in the hypodermis of C. elegans embryos during dorsal intercalation (bottom right), enclosure (top left) and elongation (top right and bottom left)
Cell junction geometry changes during elongation as shown here with AJM-1::GFP at two time points 27min. apart
During ventral enclosure epidermal cells wrap around the embryo from the dorsal side to meet at the ventral midline. In this movie hypodermal cells are expressing an actin binding GFP reporter.
Elongation involves dramatic cell shape change in all cells, including the hypodermal cells. Cell-cell junctions are highlighted in this movie of DLG-1::GFP
Gastrulation cleft closure is seen in this embryo, which is expressing a CED-10::GFP bound to all membranes.
Microtubule dynamics in a comma stage embryo can be appreciated in this 20 second interval movie of tubulin::GFP
GFP can also be expressed in the nuclei allowing the following of nuclear dynamics in developing embryos