The Cadherin Adhesome [Completed Project]

Previously, we successfully constructed the integrin adhesome, which is a database of all known components of integrin mediated adhesion and the interactions between them. We have gone on to analyze the adhesome network using systems biology approaches. We performed a similar literature based construction of a network of adherens junction components, we named the Cadhesome. The first version of our literature-based cadhesome was published in JCS in January 2013.

We went on to use proximity ligation (BioID) followed by mass spectrometry to uncover many new proteins that associate with E-cadherin at cell-cell junctions (or independently of cell-cell adhesion). Moreover, by tagging identified proteins with GFP, we determined the subcellular localization of 83 putative E-cadherin–proximal proteins and identified 24 proteins that were previously uncharacterized as part of adherens junctions. The following figure depicts the resulting comprehensive E-cadherin interaction network of 79 published and 394 previously uncharacterized proteins. This work was published in Science Signaling 7(354), rs7.

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